CD8B antibody

Catalog No. ABIN118499

Product Details anti-CD8B Antibody

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8B antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Specificity: This antibody recognises the CD8 cell surface antigen. The antibody binds to the beta chain, recognising an epitope that is dependent upon expression of both CD8 alpha and CD8 beta. Reacts with Cynomolgus Monkey.

Purification: Purified

Immunogen: Normal Human T lymphocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Clone: 2ST8-5H7

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD8B antibody (ABIN118499)

Heigele, Schindler, Gnanadurai, Leonard, Collins, Kirchhoff: "Down-modulation of CD8αβ is a fundamental activity of primate lentiviral Nef proteins." in: Journal of virology, Vol. 86, Issue 1, pp. 36-48, (2012) (PubMed).

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8 beta (CD8B Products)

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain

Gene ID: 926

NCBI Accession: NP_001171571

UniProt: P10966

Pathways: TCR Signaling