CD4 antibody
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- Target See all CD4 Antibodies
- CD4
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD4 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody recognises CD4. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognise an epitope within domain 1, of the extracellular region, of the CD4 Molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation. We recommend the use of SM1058LE for this purpose.
- Purification
- Affinity chromatography on Protein G
- Immunogen
- Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Clone
- RPA-T4
- Isotype
- IgG1
- Top Product
- Discover our top product CD4 Primary Antibody
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anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) antibodyCD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (AA 301-400) antibodyCD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE
anti-CD4 (CD4) antibody (PE)CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE
anti-CD4 (CD4) (AA 26-200) antibodyCD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on frozen sections: 1/10 - 1/100. Recommended positive control: Human lymphatic tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD4
- Alternative Name
- CD4 (CD4 Products)
- Background
- CD4 is a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
- Gene ID
- 920
- NCBI Accession
- NP_000607
- UniProt
- P01730
- Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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