MHC Class II RT-1B antibody

Catalog No. ABIN118332

Product Details anti-MHC Class II RT-1B Antibody

Target: MHC Class II RT-1B

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II RT-1B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Rat thymocyte membrane glycoproteins

Clone: OX-6

Isotype: IgG1

Application Details

Application Notes: Flow cytometry (1:50 - 1:100): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. This product is routinely tested in flow cytometry on rat splenocytes. Immunohistochemistry on frozen tissues and paraffin embedded sections (1:50 - 1:100): this clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde) (4). This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for MHC Class II RT-1B

Target: MHC Class II RT-1B

Alternative Name: MHC Class II RT1B (MHC Class II RT-1B Products)

Background: Synonyms: HLA Class II, MHC Class 2 RT1B