CD19 antibody (PE)
-
- Target See all CD19 Antibodies
- CD19 (CD19 Molecule (CD19))
-
Reactivity
- Mouse
-
Host
- Rat
-
Clonality
- Monoclonal
-
Conjugate
- This CD19 antibody is conjugated to PE
-
Application
- Flow Cytometry (FACS)
- Specificity
- This antibody recognises the CD19 cell surface antigen, a 95 kDa glycoprotein expressed by B lymphocytes. Clone 6D5 has been shown to recognise the same epitope as clone ID3 in cross-competition assays.
- Purification
- Affinity chromatography on Protein G
- Immunogen
- Human K562 cell line transfected with murine CD19. Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
- Clone
- 6D5
- Isotype
- IgG2a
- Top Product
- Discover our top product CD19 Primary Antibody
-
-
- Application Notes
-
Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Reconstitution
- Restore with 1 mL distilled water
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- This product is photosensitive and should be protected from light
- Storage
- 4 °C
- Storage Comment
- Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
-
- Target
- CD19 (CD19 Molecule (CD19))
- Alternative Name
- CD19 (CD19 Products)
- Background
- CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 Molecule is expressed on 100 % of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10 % of normal human peripheral blood cells and approximately 60 % of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12 % of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.Synonyms: B-cell marker, B-lymphocyte surface antigen B4, Differentiation antigen CD19, Leu-12
- Gene ID
- 12478
- NCBI Accession
- NP_033974
- UniProt
- P25918
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
-