CD19 antibody (Biotin)
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- Target See all CD19 Antibodies
- CD19 (CD19 Molecule (CD19))
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This CD19 antibody is conjugated to Biotin
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Application
- Flow Cytometry (FACS)
- Specificity
- This antibody recognises the CD19 cell surface antigen, a 95 kDa glycoprotein expressed by B lymphocytes. Clone 6D5 has been shown to recognise the same epitope as clone ID3 in cross-competition assays.
- Purification
- Affinity Chromatography on Protein G.
- Immunogen
- Human K562 cell line transfected with murine CD19. Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
- Clone
- 6D5
- Isotype
- IgG2a
- Top Product
- Discover our top product CD19 Primary Antibody
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- Application Notes
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Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD19 (CD19 Molecule (CD19))
- Alternative Name
- CD19 (CD19 Products)
- Background
- CD19 is a 95 kD glycoprotein, also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81 forms a molecular complex integral to B cell activation.Synonyms: B-cell marker, B-lymphocyte surface antigen B4, Differentiation antigen CD19, Leu-12
- Gene ID
- 12478
- NCBI Accession
- NP_033974
- UniProt
- P25918
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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