CD11b antibody (FITC)
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- Target See all CD11b (ITGAM) Antibodies
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
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Reactivity
- Human, Mouse, Rabbit
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This CD11b antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS)
- Purification
- Affinity chromatography on Protein A
- Immunogen
- T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.
- Clone
- M1-70-15
- Isotype
- IgG2b
- Top Product
- Discover our top product ITGAM Primary Antibody
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- Application Notes
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Flow cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
- Alternative Name
- CD11b (ITGAM Products)
- Background
- MAC-1 is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
- Gene ID
- 10090
- UniProt
- P05555
- Pathways
- Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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