ATG7 antibody (AA 558-569)

Catalog No. ABIN118061

Product Details anti-ATG7 Antibody

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Binding Specificity: AA 558-569

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Apg7 protein. However, BLAST analysis indicates 100 % homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, and chicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100 % identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.

Purification: Immunoaffinity chromatography

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 558-569 of Human Apg7 protein.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 15,000-1: 75,000) and byWestern blot (1: 500- 1: 2,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 78 kDa in size corresponding to human Apg7 bywestern blotting in the appropriate cell lysate or extract. Human Apg7 has been reported toform a homodimer at approximately 160 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.46 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

References for anti-ATG7 antibody (ABIN118061)

Aoki, Kobayashi, Tanida, Hatano, Komori, Matsumoto, Nishioka, Uemura: "[A difficult case of esophageal and gastric double cancer with pleural and pericardial effusion following chemo-radiotherapy (CRT)]" in: Gan to kagaku ryoho. Cancer & chemotherapy, Vol. 34, Issue 12, pp. 1979-81, (2008) (PubMed).

Fu, Yuan, Sun, Cui: "[Relation between acute respiratory infection and enterovirus in children in Beijing area.]" in: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology, Vol. 21, Issue 4, pp. 316-8, (2008) (PubMed).

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: APG7L / ATG7 (ATG7 Products)

Background: Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.Synonyms: APG7-like, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein

Gene ID: 10533

NCBI Accession: NP_001129503

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy