MAD2L2 antibody (AA 3-14)

Catalog No. ABIN117980

Product Details anti-MAD2L2 Antibody

Target: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Binding Specificity: AA 3-14

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAD2L2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: MAD2L2

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 3-14 of Human MAD2L2.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 7,500-1: 30,000) againstthe immunizing peptide and by Western blotting (1: 500-1: 2,000) against HeLa whole celllysates and nuclear extracts. Reactivity in other immunoassays is unknown. Antibodyactivity can be blocked using a control peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.3 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for MAD2L2

Target: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Alternative Name: MAD2L2 (MAD2L2 Products)

Background: MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.Synonyms: MAD2B, Mitotic spindle assembly checkpoint protein MAD2B, REV7

Gene ID: 10459

NCBI Accession: NP_001120797

UniProt: Q9UI95