Cullin 3 antibody (N-Term)

Catalog No. ABIN117949

Product Details anti-Cullin 3 Antibody

Target: Cullin 3 (CUL3)

Binding Specificity: AA 1-12, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequence: Human Cullin 3 (768 aa 88.9 kDa): 1 msnlskgtgs rkdtkmrira fpmtmdekyv nsiwdllkna iqeiqrknns glsfeelyrn 61 aytmvlhkhg eklytglrev vtehlinkvr edvlnslnnn flqtlnqawn dhqtamvmir 121 dilmymdrvy vqqnnvenvy nlgliifrdq vvrygcirdh lrqtlldmia rerkgevvdr 181 gairnacqml milglegrsv yeedfeapfl emsaeffqme sqkflaensa svyikkvear 241 ineeiervmh cldksteepi vkvverelis khmktiveme nsglvhmlkn gktedlgcmy 301 klfsrvpngl ktmcecmssy lreqgkalvs eegegknpvd yiqglldlks rfdrfllesf 361 nndrlfkqti agdfeyflnl nsrspeylsl fiddklkkgv kglteqevet ildkamvlfr 421 fmqekdvfer yykqhlarrl ltnksvsdds eknmisklkt ecgcqftskl egmfrdmsis 481 nttmdefrqh lqatgvslgg vdltvrvltt gywptqsatp kcnippaprh afeifrrfyl 541 akhsgrqltl qhhmgsadln atfygpvkke dgsevgvgga qvtgsntrkh ilqvstfqmt 601 ilmlfnnrek ytfeeiqqet diperelvra lqslacgkpt qrvltkepks keienghift 661 vndqftsklh rvkiqtvaak qgesdperke trqkvdddrk heieaaivri mksrkkmqhn 721 vlvaevtqql karflpspvv ikkrieglie reylartped rkvytyva

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 3. Cross reactivity is expected against mouse Cul3 based on sequence homology. Cross reactivity with other human cullins is unlikely based on sequence homology.

Purification: Delipidation and defibrination.

Immunogen: Synthetic peptide corresponding to amino acids 1-12 of Human Cul3 (N-terminus) coupled to KLH.

Application Details

Application Notes: Western blot: This antibody reacts with human Cul3 (1/500-1/1,000, An 88.9 kDa bandcorresponding to human Cul3 is detected, Most cell lines expressing Cul3 can be used as apositive control).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85.0 mg/mL (by Refractometry)

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for up to one month or at -20 °C for longer.

References for anti-Cullin 3 antibody (ABIN117949)

Chuang, Huang, Hsieh, Jung, Ye, Du, Lu, Cho, Hsiao, Hsu, Lin: "Sporadic paediatric and adult Burkitt lymphomas share similar phenotypic and genotypic features." in: Histopathology, Vol. 52, Issue 4, pp. 427-35, (2008) (PubMed).

Target Details for Cullin 3

Target: Cullin 3 (CUL3)

Alternative Name: Cullin-3 (CUL3 Products)

Background: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.Synonyms: CUL-3, CUL3, KIAA0617

Gene ID: 8452

NCBI Accession: NP_003581

UniProt: Q13618

Pathways: M Phase