GFP antibody (HRP)

Catalog No. ABIN117894

Product Details anti-GFP Antibody (HRP)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This product was prepared from tissue culture supernatant by Protein A chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. Reactivity is observed against wild type, recombinant and enhanced forms of GFP.

Purification: Protein A chromatography

Immunogen: The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria

Clone: 9F9-F9

Isotype: IgG2a

Application Details

Application Notes: Designed to detect GFP and its variants in ELISA (sandwich or capture), Immunoblottingand Immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISAfor direct binding of antigen recognize wild type, recombinant and enhanced forms of GFP. Monoclonal and polyclonal forms anti-GFP assayed in a sandwich ELISA are well suited totitrate GFP in solution using either form of the antibody as the capture or detection

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Thimerisol as preservative and 10 mg/mL Bovine Serum Albumin (BSA) (IgG and Protease free) as stabilizer

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

References for anti-GFP antibody (HRP) (ABIN117894)

Taheri, Seyed, Rafati: "DNA Integration in Leishmania Genome: An Application for Vaccine Development and Drug Screening." in: Methods in molecular biology (Clifton, N.J.), Vol. 1403, pp. 603-22, (2016) (PubMed).

Luijsterburg, Goedhart, Moser, Kool, Geverts, Houtsmuller, Mullenders, Vermeulen, van Driel: "Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC." in: Journal of cell science, Vol. 120, Issue Pt 15, pp. 2706-16, (2007) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein antibody, gfp antibody

Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein

Gene ID: 6100

UniProt: P42212