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CD3 antibody

Name: CD3 antibody
SKU: ABIN1177372
Availability: OutOfStock

CD3 Reactivity: Human FACS, Func Host: Mouse Monoclonal UCHT1 unconjugated

FACS

1 image

(1 reference)

Catalog No. ABIN1177372

Datasheet as PDF

Target See all CD3 Antibodies

CD3
Reactivity
183

104

26

20

17

10

8

8

7

7

5

3

2

1
Human
Host
218

53

20

13

9

2
Mouse
Compatible Secondaries
Clonality
303

6

1
Monoclonal
Conjugate
96

39

32

23

23

11

9

8

6

5

5

5

4

4

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is un-conjugated
Application
288

82

58

52

46

37

33

23

9

8

7

6

4

3

2

2

2

2

2

2

2

2

1

1

1
Flow Cytometry (FACS), Functional Studies (Func)

Brand

BD Pharmingen™

Purification

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility

0.2 μm filtered

Endotoxin Level

Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Clone

UCHT1

Isotype

IgG1 kappa

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anti-CD3 (CD3) antibody
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro), ICC, CyTOF Host: Mouse Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human FACS, IP, CyTOF Host: Mouse Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human FACS, IP, Func, CyTOF Host: Mouse Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF Host: Mouse Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-57 Biotin

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro) Host: Mouse Monoclonal UCHT1 Biotin

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PerCP

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human FACS Host: Mouse Monoclonal MEM-57 PerCP

anti-CD3 (CD3) antibody (APC)
CD3 Reactivity: Human FACS Host: Mouse Monoclonal MEM-57 APC

Restrictions

For Research Use only
Format

Liquid

Concentration

1.0 mg/mL

Buffer

No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative

Azide free

Storage

4 °C

Storage Comment

Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Beverley, Callard: "Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody." in: European journal of immunology, Vol. 11, Issue 4, pp. 329-34, (1981) (PubMed).
Target

CD3

Alternative Name

CD3 (CD3 Products)

Background

The UCHT1 monoclonal antibody specifically binds to the human CD3epsilon-chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3epsilon is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3epsilon-positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3epsilon unlike the other CD3 clone, HIT3a, that stains only extracellular CD3epsilon.
Synonyms: CD3e, CD3E, T3E, TCRE , T-cell surface antigen T3/Leu-4 epsilon

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway