CD3 antibody

Catalog No. ABIN1177371

Product Details anti-CD3 Antibody

Target: CD3

Reactivity: Rhesus Monkey, Cynomolgus, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Clone: SP34

Isotype: IgG3 lambda

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD3 antibody (ABIN1177371)

Sancho, Ledbetter, Choi, Kanner, Deans, Terhorst: "CD3-zeta surface expression is required for CD4-p56lck-mediated upregulation of T cell antigen receptor-CD3 signaling in T cells." in: The Journal of biological chemistry, Vol. 267, Issue 11, pp. 7871-9, (1992) (PubMed).

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Background: Clone SP34 reacts with human form of the 20 kDa epsilon subunit of the CD3 antigen/T-cell receptor (TCR) complex. This clone also cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of rhesus or cynomolgus and pigtail macaque monkeys. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes, with the majority of CD3-positive cells being negative when dual stained with antibodies to B or NK cell markers.

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway