CD3 antibody
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- Target See all CD3 Antibodies
- CD3
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD3 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Functional Studies (Func)
- Brand
- BD Pharmingen™
- Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- Clone
- HIT3a
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product CD3 Primary Antibody
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- Preservative
- Azide free
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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- Target
- CD3
- Alternative Name
- CD3 (CD3 Products)
- Background
- Reacts with the human epsilon-chain, a 20 kDa subunit of CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show this antibody to be mitogenic when used in conjuction with pokeweed mitogen. CD3 plays a role in signal transduction during antigen recognition. HIT3a antibody does not stain intracellular CD3 unlike the other CD3 clone, UCHT1.
- Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
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