CD81 antibody

Catalog No. ABIN1177362

Product Details anti-CD81 Antibody

Target: CD81

Reactivity: Mouse, Rat

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Clone: Eat2

Isotype: IgG kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD81 antibody (ABIN1177362)

Deng, Yeung, Tsitoura, DeKruyff, Umetsu, Levy: "Allergen-induced airway hyperreactivity is diminished in CD81-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 5054-61, (2000) (PubMed).

Maecker, Todd, Kim, Levy: "Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies." in: Hybridoma, Vol. 19, Issue 1, pp. 15-22, (2000) (PubMed).

Levy, Todd, Maecker: "CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system." in: Annual review of immunology, Vol. 16, pp. 89-109, (1998) (PubMed).

Maecker, Do, Levy: "CD81 on B cells promotes interleukin 4 secretion and antibody production during T helper type 2 immune responses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 5, pp. 2458-62, (1998) (PubMed).

Fleming, Donnadieu, Song, Laethem, Galli, Kinet: "Negative regulation of Fc epsilon RI-mediated degranulation by CD81." in: The Journal of experimental medicine, Vol. 186, Issue 8, pp. 1307-14, (1997) (PubMed).

Maecker, Levy: "Normal lymphocyte development but delayed humoral immune response in CD81-null mice." in: The Journal of experimental medicine, Vol. 185, Issue 8, pp. 1505-10, (1997) (PubMed).

Miyazaki, Müller, Campbell: "Normal development but differentially altered proliferative responses of lymphocytes in mice lacking CD81." in: The EMBO journal, Vol. 16, Issue 14, pp. 4217-25, (1997) (PubMed).

Tsitsikov, Gutierrez-Ramos, Geha: "Impaired CD19 expression and signaling, enhanced antibody response to type II T independent antigen and reduction of B-1 cells in CD81-deficient mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 20, pp. 10844-9, (1997) (PubMed).

Boismenu, Rhein, Fischer, Havran: "A role for CD81 in early T cell development." in: Science (New York, N.Y.), Vol. 271, Issue 5246, pp. 198-200, (1996) (PubMed).

Tedder, Zhou, Engel: "The CD19/CD21 signal transduction complex of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 437-42, (1994) (PubMed).

Target Details for CD81

Target: CD81

Alternative Name: CD81 (CD81 Products)

Background: The Eat2 monoclonal antibody specifically binds to CD81, a 26-kDa nonglycosylated member of the transmembrane 4 integral membrane protein superfamily, expressed by many types of cells. For example, CD81 participates with CD19 and CD21 in the signal transduction complex associated with the B-cell receptor on human B lymphocytes and with the CD4 and CD8 co-receptors on human thymocytes and T lymphocytes. In mouse fetal thymic organ culture, interactions of immature thymocytes with CD81 expressed by thymic stromal cells are required to induce development of T cells with alphabeta T-cell receptors. Furthermore, CD81 has been shown to play a role in the regulation of rat mastcell degranulation. Despite its important roles in the immune response and wide tissue distribution, CD81-deficient mice are born without obvious developmental abnormalities. However, these mice have abnormal immune responses, and impaired fertility. Eat2 mAb cross-reacts with the rat CD81 antigen. Eat2 mAb was reported cross-reacting with the rat CD81 antigen.
Synonyms: Tspan28, Tapa1, Tapa-1, Target of the antiproliferative antibody 1, Trpm5

Pathways: Inositol Metabolic Process, Hepatitis C