Interleukin 17a antibody

Catalog No. ABIN1177292

Product Details anti-Interleukin 17a Antibody

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Interleukin 17a antibody is un-conjugated

Application: ELISA, Neutralization (Neut), Intracellular Staining (ICS)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Recombinant Mouse IL-17A Protein

Clone: TC11-18H10

Isotype: IgG1 kappa

Application Details

Protocol: This procedure is to be used for blocking, it is not routinely tested for each batch. 1. In the appropriate tissue culture medium dilute TC11-18H10 NA/LE mAb with the highest concentration of 12 µg/ml at a 1:4 series dilution in the tissue culture hood for a total of 7 different concentrations.
2. Add 50 µl into each well. For the negative control, add 50 µl tissue culture medium.
3. Set the triplicate for each testing concentration of TC11-18H10 NA/LE antibody . 560268 4. Dilute the rmIL-17A in the tissue culture medium to 40 ng/ml. Add 50 µl into each well. Tap the plate to mix the mAb and rmIL-17A together.
5. Incubate the plate in 37°C, 5% CO2 incubator for 1 hour.
6. Count the log phase NIH-3T3 cells and dilute the cells to 5x10^5 cell/ml. Seed 100 µl cells into each well.
7. Incubate the cells in 37°C, 5% CO2 incubator for 24 hours. 8. Harvest the supernantant. Check the IL-6 concentration from each well using the BD Cytometric Bead Array Mouse IL-6 Flex Set.
9. Calculate the IL-6 concentration of each well from the standard curve. Calculate the mean of each concentration triplicate and plot a chart

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-Interleukin 17a antibody (ABIN1177292)

Yen, Cheung, Scheerens, Poulet, McClanahan, McKenzie, Kleinschek, Owyang, Mattson, Blumenschein, Murphy, Sathe, Cua, Kastelein, Rennick: "IL-23 is essential for T cell-mediated colitis and promotes inflammation via IL-17 and IL-6." in: The Journal of clinical investigation, Vol. 116, Issue 5, pp. 1310-6, (2006) (PubMed).

Schwarzenberger, La Russa, Miller, Ye, Huang, Zieske, Nelson, Bagby, Stoltz, Mynatt, Spriggs, Kolls: "IL-17 stimulates granulopoiesis in mice: use of an alternate, novel gene therapy-derived method for in vivo evaluation of cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 6383-9, (1998) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL-17A (IL17A Products)

Background: The TC11-18H10 antibody reacts with recombinant and natural mouse IL-17A proteins. IL-17A, also known as CTLA-8, is a T cell-derived cytokine that promotes inflammatory responses. Mouse IL-17A is a proinflammatory cytokine that can induce the release of IL-6 by mouse stromal cells. It has been shown to support the growth of hemopoietic progenitors in vitro, it can also stimulate granulopoiesis in vivo. The TC11-18H10 antibody has been reported to neutralize IL-17A activity. Recent studies have shown that IL-17A is produced by a unique subset of Th17 cells that develop along a pathway distinct from the Th1- and Th2- cell differentiation pathways. The mouse IL-17A cDNA was isolated from a cDNA library generated from TCRalphabeta+CD4-CD8- thymocytes. Functional studies of TC11-18H10 mAb by neutralization of mouse IL-17A bioactivity. Recombinant mouse IL-17A (2 ng/well) was pre-incubated with various amounts of TC11-18H10 mAb or medium as a control in a 96-well plate for 1 hour at 37°C. Following the incubation, NIH-3T3 cells were added at 5x10^4 cells per well and incubated at 37°C. After 24 hours of incubation, the supernatant was harvested and the concentration of IL-6 was quantified using BD™ Cytometric Bead Array Mouse IL-6 Flex Set (Cat. No. 558301).
Synonyms: Interleukin-17A, Il17a, Cytotoxic T-lymphocyte-associated antigen, CTLA-8