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Goat anti-Human Ig (Chain kappa), (Light Chain) Antibody (FITC) - Preadsorbed

FACS, IF, IM Polyclonal FITC F(ab')2 fragment
Catalog No. ABIN117714
  • Target See all Ig products
    Ig
    Fragment
    F(ab')2 fragment
    Binding Specificity
    • 88
    • 86
    • 68
    • 32
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    Chain kappa, Light Chain
    Reactivity
    • 108
    • 61
    • 26
    • 17
    • 9
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 98
    • 73
    • 36
    • 20
    • 2
    • 1
    • 1
    • 1
    Goat
    Clonality
    • 111
    • 95
    Polyclonal
    Conjugate
    • 59
    • 29
    • 22
    • 21
    • 17
    • 16
    • 10
    • 10
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Application
    • 162
    • 125
    • 86
    • 67
    • 41
    • 26
    • 25
    • 14
    • 12
    • 9
    • 8
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunomicroscopy (IM)
    Characteristics
    Absorption / Emission: 495 nm / 528 nm
    Molar Ratio: 5.9 moles FITC per mole of Goat IgG F(ab')2.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen
    Human kappa (kappa) light chain
  • Application Notes
    Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence ofF(c) mediated binding, lot-to-lot consistency, high titer and specificity.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1.0 mL of deionized water (or equivalent).
    Concentration
    1.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
  • Target
    Ig
    Alternative Name
    Immunoglobulin (Ig Products)
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