Goat anti-Human Ig (Chain kappa), (Light Chain) Antibody (FITC) - Preadsorbed
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- Target See all Ig products
- Ig
- Fragment
- F(ab')2 fragment
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Binding Specificity
- Chain kappa, Light Chain
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- FITC
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunomicroscopy (IM)
- Characteristics
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Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 5.9 moles FITC per mole of Goat IgG F(ab')2. - Purification
- Preadsorption: Solid phase absorption
- Immunogen
- Human kappa (kappa) light chain
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- Application Notes
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Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence ofF(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1.0 mL of deionized water (or equivalent).
- Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Target
- Ig
- Alternative Name
- Immunoglobulin (Ig Products)
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