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Goat anti-Human Ig (Chain kappa), (Light Chain) Antibody (Biotin)

ELISA, IHC, WB, IEP, IM Polyclonal Biotin F(ab')2 fragment

Catalog No. ABIN117713

Datasheet as PDF

Target See all Ig products

Ig

Fragment

F(ab')2 fragment
Binding Specificity
88

86

68

32

4

4

2

1

1

1
Chain kappa, Light Chain
Reactivity
108

61

26

17

9

4

2

1

1

1

1

1

1

1

1

1
Human
Host
98

73

36

20

2

1

1

1
Goat
Clonality
111

95
Polyclonal
Conjugate
59

29

22

21

17

16

10

10

5

4

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
Biotin
Application
162

125

86

67

41

26

25

14

12

9

8

6

3

2

2

1

1

1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoelectrophoresis (IEP), Immunomicroscopy (IM)

Characteristics

Molar Ratio: 10-20 BAC molecules per Goat IgG Fab molecule.

Purification

Immunoaffinity chromatography.

Immunogen

Human kappa (kappa) light chain.
Goat anti-Human Ig (Chain kappa) antibody (Biotin)
ELISA, FACS, IHC, WB Polyclonal IgG Biotin

Goat anti-Human Ig antibody
ELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment

Goat anti-Human Ig antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Human Ig antibody (FITC)
ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment

Goat anti-Human Ig (Heavy & Light Chain) antibody (Biotin)
ELISA, FACS, WB Polyclonal IgG Biotin

Goat anti-Human Ig (Heavy & Light Chain) antibody (HRP)
ELISA, WB Polyclonal IgG HRP

Goat anti-Human Ig (Chain lambda) antibody
ELISA, FACS, IHC, WB Polyclonal IgG unconjugated

Goat anti-Human Ig antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Goat anti-Human Ig (Chain lambda) antibody (Texas Red (TR))
ELISA, FACS, IHC Polyclonal IgG Texas Red (TR)

Goat anti-Human Ig (Chain lambda) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Application Notes

Suitable for use in Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy aswell as other antibody based assays using streptavidin or avidin conjugates requiringextremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency,high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 8,500 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Reconstitution

Restore with 1.0 mL of deionized water (or equivalent).

Concentration

1.0 mg/mL (by UV absorbance at 280 nm)

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage

4 °C/-20 °C

Storage Comment

Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Target

Ig

Alternative Name

Immunoglobulin (Ig Products)