CD3 antibody
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- Target See all CD3 Antibodies
- CD3
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD3 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Biochemical Assay (BCA)
- Brand
- BD Pharmingen™
- Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- Immunogen
- PHA-stimulated rat lymph node and spleen cells
- Clone
- G4-18
- Isotype
- IgG3 kappa
- Top Product
- Discover our top product CD3 Primary Antibody
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2μm sterile filtered.
- Preservative
- Azide free
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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Regulation of T-cell activation in the lung: alveolar macrophages induce reversible T-cell anergy in vitro associated with inhibition of interleukin-2 receptor signal transduction." in: Immunology, Vol. 87, Issue 2, pp. 250-8, (1996) (PubMed).
: "Alloreactive natural killer cells in the rat: complex genetics of major histocompatibility complex control." in: European journal of immunology, Vol. 25, Issue 5, pp. 1249-56, (1995) (PubMed).
: "Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion." in: Immunology, Vol. 84, Issue 1, pp. 142-7, (1995) (PubMed).
: "Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody." in: Transplantation, Vol. 55, Issue 3, pp. 459-68, (1993) (PubMed).
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Regulation of T-cell activation in the lung: alveolar macrophages induce reversible T-cell anergy in vitro associated with inhibition of interleukin-2 receptor signal transduction." in: Immunology, Vol. 87, Issue 2, pp. 250-8, (1996) (PubMed).
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- Target
- CD3
- Alternative Name
- CD3 (CD3 Products)
- Background
- The G4.18 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. It has been reported that CD3 expression is down-regulated within 24 hours in concanavalin A-stimulated rat T cells, and soluble mAb inhibits the allogeneic mixed-lymphocyte proliferative response and cell-mediated cytotoxicity to allogeneic target cells. In vivo treatment with G4.18 mAb prevents cardiac and skin allograft rejection, resulting in donor-specific tolerance. Pre-incubation of splenocytes with the alternate anti-rat CD3 monoclonal antibody, 1F4 (Cat. No. 556970), blocks staining with mAb G4.18.
- Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
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