CD90.2 / Thy-1.2 antibody

Catalog No. ABIN1177007

Product Details anti-CD90.2 / Thy-1.2 Antibody

Target: CD90.2 / Thy-1.2

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD90.2 / Thy-1.2 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse Thymus / Spleen

Clone: 30-H12

Isotype: IgG2b kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD90.2 / Thy-1.2 antibody (ABIN1177007)

Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, (1997) (PubMed).

Borrello, Phipps: "Differential Thy-1 expression by splenic fibroblasts defines functionally distinct subsets." in: Cellular immunology, Vol. 173, Issue 2, pp. 198-206, (1996) (PubMed).

Radrizzani, Carminatti, Pivetta, Idoyaga Vargas: "Developmental regulation of Thy 1.2 rate of synthesis in the mouse cerebellum." in: Journal of neuroscience research, Vol. 42, Issue 2, pp. 220-7, (1996) (PubMed).

Zhong, Donnenberg, Edison, Harrison: "The appearance of Thy-1- donor T cells in the peripheral circulation 3-6 weeks after bone marrow transplantation suggests an extrathymic origin." in: International immunology, Vol. 8, Issue 2, pp. 171-6, (1996) (PubMed).

Wu, Vremec, Ardavin, Winkel, Süss, Georgiou, Maraskovsky, Cook, Shortman: "Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation." in: European journal of immunology, Vol. 25, Issue 2, pp. 418-25, (1995) (PubMed).

Hueber, Raposo, Pierres, He: "Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism." in: The Journal of experimental medicine, Vol. 179, Issue 3, pp. 785-96, (1994) (PubMed).

Nakashima, Pu, Hamaguchi, Iwamoto, Rahman, Zhang, Kato, Ohkusu, Katano, Yoshida: "Pathway of signal delivery to murine thymocytes triggered by co-crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3511-20, (1993) (PubMed).

Ikuta, Uchida, Friedman, Weissman: "Lymphocyte development from stem cells." in: Annual review of immunology, Vol. 10, pp. 759-83, (1992) (PubMed).

Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, (1992) (PubMed).

He, Naquet, Caillol, Pierres: "Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 515-8, (1991) (PubMed).

Nakashima, Zhang, Rahman, Yoshida, Isobe, Ding, Iwamoto, Hamaguchi, Ikezawa, Taguchi: "Evidence of synergy between Thy-1 and CD3/TCR complex in signal delivery to murine thymocytes for cell death." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 4, pp. 1153-62, (1991) (PubMed).

Tigelaar, Lewis, Bergstresser: "TCR gamma/delta+ dendritic epidermal T cells as constituents of skin-associated lymphoid tissue." in: The Journal of investigative dermatology, Vol. 94, Issue 6 Suppl, pp. 58S-63S, (1990) (PubMed).

Kroczek, Gunter, Germain, Shevach: "Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes." in: Nature, Vol. 322, Issue 6075, pp. 181-4, (1986) (PubMed).

Williams, Gagnon: "Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin." in: Science (New York, N.Y.), Vol. 216, Issue 4547, pp. 696-703, (1982) (PubMed).

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, (1980) (PubMed).

Ledbetter, Rouse, Micklem, Herzenberg: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, (1980) (PubMed).

Target Details for CD90.2 / Thy-1.2

Target: CD90.2 / Thy-1.2

Alternative Name: CD90.2 (CD90.2 / Thy-1.2 Products)

Background: The 30-H12 clone has been reported to react with the CD90.2 (Thy-1.2) alloantigen on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains. Thy-1.2 has also been initially reported to be detectable on thymic dendritic cells, but later revealed that the antigen was probably picked up from T-lineage cells. 30-H12 mAb has been reported not to cross-react with Thy-1.1 (e.g., AKR/J, PL), or with rat Thy-1. CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma. It has been reported that crosslinked 30-H12 antibody induces Ca2+ influx into thymocytes and that co-crosslinking of 30-H12 mAb with antibody to the CD3/TCR complex intensifies thymocyte signal transduction, promotes apoptosis of thymocytes, and inhibits the CD3-mediated proliferative response of mature T lymphocytes.
Synonyms: Thy-1.2