CD9 antibody

Catalog No. ABIN1176843

Product Details anti-CD9 Antibody

Target: CD9

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal cell line BMS2

Clone: KMC8

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD9 antibody (ABIN1176843)

Nakorn, Miyamoto, Weissman: "Characterization of mouse clonogenic megakaryocyte progenitors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 1, pp. 205-10, (2003) (PubMed).

Kaji, Takeshita, Miyake, Takai, Kudo: "Functional association of CD9 with the Fc gamma receptors in macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3256-65, (2001) (PubMed).

Tachibana, Hemler: "Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance." in: The Journal of cell biology, Vol. 146, Issue 4, pp. 893-904, (1999) (PubMed).

Tanio, Yamazaki, Kunisada, Miyake, Hayashi: "CD9 molecule expressed on stromal cells is involved in osteoclastogenesis." in: Experimental hematology, Vol. 27, Issue 5, pp. 853-9, (1999) (PubMed).

Park, Yashiro, Tai, Toyo-oka, Hamaoka, Yagita, Okumura, Neben, Fujiwara: "Differential involvement of a Fas-CPP32-like protease pathway in apoptosis of TCR/CD9-costimulated, naive T cells and TCR-restimulated, activated T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 5790-6, (1998) (PubMed).

Pulendran, Lingappa, Kennedy, Smith, Teepe, Rudensky, Maliszewski, Maraskovsky: "Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2222-31, (1997) (PubMed).

Oritani, Wu, Medina, Hudson, Miyake, Gimble, Burstein, Kincade: "Antibody ligation of CD9 modifies production of myeloid cells in long-term cultures." in: Blood, Vol. 87, Issue 6, pp. 2252-61, (1996) (PubMed).

Wright, Tomlinson: "The ins and outs of the transmembrane 4 superfamily." in: Immunology today, Vol. 15, Issue 12, pp. 588-94, (1995) (PubMed).

Jennings, Crossno, Fox, White, Green: "Platelet p24/CD9, a member of the tetraspanin family of proteins." in: Annals of the New York Academy of Sciences, Vol. 714, pp. 175-84, (1994) (PubMed).

Miyake, Medina, Hayashi, Ono, Hamaoka, Kincade: "Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures." in: The Journal of experimental medicine, Vol. 171, Issue 2, pp. 477-88, (1990) (PubMed).

Target Details for CD9

Target: CD9

Alternative Name: CD9 (CD9 Products)

Background: The KMC8 antibody reacts with CD9, a 24-kDa member of the transmembrane 4 superfamily. In the mouse, CD9 is present on bone marrow myeloid cells, stromal cells, and megakaryocyte-committed progenitors, subsets of peripheral T and B lymphocytes, and neutrophils, platelets, dendritic cells, and bone marrow-derived macrophages. CD9 has been found to be associated with integrins and other cell-surface receptors, and it is suggested to play a role in signal transduction and possibly in regulating cellular adhesive properties. It has also been demonstrated to participate in T-cell costimulation and induction of apoptosis. The KMC8 antibody has been reported to block certain CD9 functions and activate macrophages

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization