Rabbit anti-Human IgG (Whole Molecule) Antibody (TRITC)
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- Target See all IgG products
- IgG
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Binding Specificity
- Whole Molecule
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- TRITC
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Application
- Flow Cytometry (FACS), Immunomicroscopy (IM)
- Characteristics
- Molar Ratio: 2.7 moles TRITC per mole of Rabbit IgG.
- Purification
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Immunoaffinity chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads. - Immunogen
- Human IgG, whole molecule.
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- Application Notes
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Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Comment
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Absorption / Emission: 550 nm / 570 nm
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1.0 mL of deionized water (or equivalent).
- Concentration
- 2.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative and 10 mg/mL BSA (IgG and Protease free) as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Product is photosensitive and should be protected from light. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Synonyms: Human Immunoglobulin G
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