Urm1 antibody

Catalog No. ABIN116960

Product Details anti-Urm1 Antibody

Target: Urm1 (Ubiquitin Related Modifier 1 (Urm1))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Urm1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This product is prepared from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.

Purification: Multi-step process.

Immunogen: Recombinant yeast Urm1 protein.

Application Details

Application Notes: Suitable for ELISA (1/2,000-1/10,000) and Immunoblotting (1/1,000). Although not tested,this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsicbands (UBLs or their conjugates).
Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. A 12 kDa band corresponding to yeast Urm1 is detected. Most yeast cell lysates can beused as a positive control without induction or stimulation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Urm1

Target: Urm1 (Ubiquitin Related Modifier 1 (Urm1))

Alternative Name: URM1 (Urm1 Products)

Background: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Urm1 is a newly identified ubiquitin related modifier. Urm 1 is a 99-amino acid protein terminated with glycine-glycine. Target proteins are conjugated to Urm1 via its C-terminal glycine. Initially Urm1 forms a thioester with a novel E1-like protein, Uba4.Synonyms: C9orf74, URM-1, Ubiquitin-related modifier 1, YIL008W

Gene ID: 854809

NCBI Accession: NP_012258

UniProt: P40554