HRP antibody (Biotin)

Catalog No. ABIN116807

Product Details anti-HRP Antibody (Biotin)

Target: HRP (Horseradish Peroxidase (HRP))

Reactivity: Horseradish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRP antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Characteristics: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule.

Purification: Multi-step process.

Immunogen: Peroxidase from Horseradish.

Application Details

Application Notes: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 10.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative and 10 mg/mL BSA (IgG and Protease free) as stabiliser.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for HRP

Target: HRP (Horseradish Peroxidase (HRP))

Alternative Name: Horseradish Peroxidase / HRP (HRP Products)