HRP antibody

Catalog No. ABIN116806

Product Details anti-HRP Antibody

Target: HRP (Horseradish Peroxidase (HRP))

Reactivity: Horseradish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRP antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Prepared from monospecific antiserum by a Delipidation and Defibrination

Immunogen: Peroxidase from Horseradish.

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot, 1/2,000-1/10,000), ELISA(1/20,000-1/100,000), Immunoprecipitation, Immunohistochemistry (1/1,000-1/5,000)and most Immunological methods requiring high titer and specificity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.01 M Sodium Phosphate, 0.11 M Sodium Chloride, pH 7.2, None, None

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use. . Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for HRP

Target: HRP (Horseradish Peroxidase (HRP))

Alternative Name: Horseradish Peroxidase / HRP (HRP Products)

Background: Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.