Collagen IV antibody (Biotin)

Catalog No. ABIN116559

Product Details anti-Collagen IV Antibody (Biotin)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Characteristics: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Purification: Immunoaffinity chromatography.

Immunogen: Collagen Type IV from human and bovine placenta.

Application Details

Application Notes: Suitable for indirect trapping ELISA for quantitation of antigen in serum using a standardcurve, for immunoprecipitation and for western blotting for highly sensitive qualitativeanalysis. Recommended Dilution(s): This product was assayed by immunoblot and found to bereactive against Collagen IV at a dilution of 1: 5,000 to 1: 10,000. This product was alsoassayed against 1.0 μg of Collagen IV in a standard sandwich ELISA using PeroxidaseConjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. A working dilution of 1: 4,000 to1: 8,000 of the stock concentration is suggested for this product. For Immunohistochemistry on frozen tissue sections dilute the product 1: 50 to 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 with 10 mg/mL BSA (Ig and Protease Free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

References for anti-Collagen IV antibody (Biotin) (ABIN116559)

Behrens, Villone, Koch, Brunner, Sorokin, Robenek, Bruckner-Tuderman, Bruckner, Hansen: "The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18700-9, (2012) (PubMed).

Sasse, Malan, Fleischmann, Roell, Gustafsson, Bostani, Fan, Kolbe, Breitbach, Addicks, Welz, Brem, Hescheler, Aszodi, Costell, Bloch, Fleischmann: "Perlecan is critical for heart stability." in: Cardiovascular research, Vol. 80, Issue 3, pp. 435-44, (2009) (PubMed).

Taylor, Gonzalez-Begne, Sojka, Richardson, Sheardown, Harrison, Pusey, Tam, Elliott: "Lymphocytes from P2X7-deficient mice exhibit enhanced P2X7 responses." in: Journal of leukocyte biology, Vol. 85, Issue 6, pp. 978-86, (2009) (PubMed).

Suzuki, Mogami, Ihara, Urano: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, (2009) (PubMed).

Hayworth, Moody, Chodosh, Krieg, Rimer, Thompson: "Induction of neuregulin signaling in mouse schwann cells in vivo mimics responses to denervation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 25, pp. 6873-84, (2006) (PubMed).

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody

Background: Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.Synonyms: COL4A1

Gene ID: 9606

UniProt: P02462