Collagen IV antibody

Catalog No. ABIN116558

Product Details anti-Collagen IV Antibody

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Purification: Immunoaffinity Chromatography.

Immunogen: Collagen type IV purified from Human and Bovine placenta.

Application Details

Application Notes: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for native(non-denaturing, non-dissociating) PAGE and Western blotting for highly sensitivequalitative analysis. Recommended Dilutions: ELISA 1/5,000-1/20,000. Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/200 (prepare tissues as frozen tissue sections). Note: This product was assayed by Immunoblot and found to be reactive against CollagenIV at a dilution of 1/5,000-1/10,000. This product was also assayed against 1.0 μg of Collagen IV in a standard sandwich ELISAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP)and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/8,000 of the stock

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

References for anti-Collagen IV antibody (ABIN116558)

Rodriguez-Teja, Gronau, Breit, Zhang, Minamidate, Caley, McCarthy, Cox, Erler, Gaughan, Darby, Robson, Mauri, Waxman, Sturge: "AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival." in: The Journal of pathology, Vol. 235, Issue 4, pp. 581-92, (2015) (PubMed).

Behrens, Villone, Koch, Brunner, Sorokin, Robenek, Bruckner-Tuderman, Bruckner, Hansen: "The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18700-9, (2012) (PubMed).

Sasse, Malan, Fleischmann, Roell, Gustafsson, Bostani, Fan, Kolbe, Breitbach, Addicks, Welz, Brem, Hescheler, Aszodi, Costell, Bloch, Fleischmann: "Perlecan is critical for heart stability." in: Cardiovascular research, Vol. 80, Issue 3, pp. 435-44, (2009) (PubMed).

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens were extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.Synonyms: COL4A1

Gene ID: 9606

UniProt: P02462