Collagen Type I antibody (Biotin)

Catalog No. ABIN116550

Product Details anti-Collagen Type I Antibody (Biotin)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human, Rat, Mouse, Cow, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This product has been prepared by Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Characteristics: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Purification: Immunoaffinity Chromatography.

Immunogen: Collagen Type I from human and bovine placenta.

Application Details

Application Notes: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended Dilutions: ELISA: 1/3,000-1/6,000. Western blot: 1/3,000-1/6,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/200.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use. . Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

References for anti-Collagen Type I antibody (Biotin) (ABIN116550)

Jagielski, Wolf, Marzahn, Völker, Lemke, Meier, Ertel, Godkin, Arens, Schulze-Tanzil: "The influence of IL-10 and TNFα on chondrogenesis of human mesenchymal stromal cells in three-dimensional cultures." in: International journal of molecular sciences, Vol. 15, Issue 9, pp. 15821-44, (2015) (PubMed).

Behrens, Villone, Koch, Brunner, Sorokin, Robenek, Bruckner-Tuderman, Bruckner, Hansen: "The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18700-9, (2012) (PubMed).

Sasse, Malan, Fleischmann, Roell, Gustafsson, Bostani, Fan, Kolbe, Breitbach, Addicks, Welz, Brem, Hescheler, Aszodi, Costell, Bloch, Fleischmann: "Perlecan is critical for heart stability." in: Cardiovascular research, Vol. 80, Issue 3, pp. 435-44, (2009) (PubMed).

Villone, Fritsch, Koch, Bruckner-Tuderman, Hansen, Bruckner: "Supramolecular interactions in the dermo-epidermal junction zone: anchoring fibril-collagen VII tightly binds to banded collagen fibrils." in: The Journal of biological chemistry, Vol. 283, Issue 36, pp. 24506-13, (2008) (PubMed).

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I (COL1 Products)

Synonyms: collagen type III alpha 1 chain antibody, COL3A1 antibody

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens for immunization from human and bovine placenta and cartilage have been extensively purified by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS PAGE and immunoblotting.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 1277

NCBI Accession: NP_000079

UniProt: P02452