CXCL1 antibody

Catalog No. ABIN116259

Product Details anti-CXCL1 Antibody

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody is specific for Human Growth Related Oncoprotein alpha. Other species not tested.

Purification: Affinity Chromatography immobilized Human GRO/MGSA matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Human GRO/MGSA (Cat.-No PA062). Remarks: Molecular Weight: ~150 kDa

Application Details

Application Notes: NeutralisationTo yield one-half maximal inhibition [ND50] of the biological activity of hGRO/MGSA 100 ng/mL), a concentration of 1.5-3.0 μg/mL of this antibody is required. Sandwich ELISATo detect Human GRO/MGSA by Sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated anti Human GRO/MGSA (PP1080B1 orPP1080B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hGRO/MGSA. Western blotTo detect Human GRO/MGSA by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGRO/MGSA is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions. Immunohistochemistry on Frozen and Paraffin Embedded Sections. A starting dilution of0.5-2 μg is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL1

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Alternative Name: CXCL1 / GRO-alpha (CXCL1 Products)

Background: Three Human MGSA/GRO genes encode 3 highly related chemokines, MGSA/GRO alpha, -beta and -gamma. All 3 MGSA/GRO proteins bind to the same receptors, but with differing affinities, and stimulate a number of biological responses including chemotaxis, angiogenesis, and growth regulation. Melanoma growth stimulatory activity/gro alpha (MGSA), a member of the alpha-chemokine family, is produced by a variety of dermal and epidermal cells and can act in a paracrine and autocrine fashion.Synonyms: CXCL1, GRO, GRO-alpha(1-73), GRO1, GROA, Growth-regulated alpha protein, MGSA, Melanoma growth stimulatory activity, NAP-3, Neutrophil-activating protein 3, SCYB1

Gene ID: 2919

NCBI Accession: NP_001502

UniProt: P09341

Pathways: Autophagy