Endoglin antibody (AA 26-586)

Catalog No. ABIN115698

Product Details anti-Endoglin Antibody

Target: Endoglin (ENG)

Binding Specificity: AA 26-586

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endoglin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Purification: Antigen-Affinity Chromatography.

Immunogen: Highly pure recombinant Human soluble CD105/Endoglin produced in Insect Cells. Remarks: It consists of amino acid 26 (Glu) to 586 (Leu) and is fused to a C-terminal His-tag (6xHis).

Application Details

Application Notes: ELISA: Use at 1-15 μg/mL. Western blot: Use at 1-2 μg/mL. Flow Cytometry: Use at 3-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile Water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.4 without preservatives and stabilizers

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105 / Endoglin (ENG Products)

Background: Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human endoglin, the S-endoglin and L-endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse endoglin share approximately 70 % and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. It has clearly been shown that CD105/Endoglin is required for angiogenesis and it plays a key role in heart development. Mutations in human endoglin or ALK-1 (another type I serine/threonine receptor) lead to the vascular disorder hereditary hemorrhagic telangiectasia (HHT). Mice heterozygous for endoglin have been developed as disease models for HHT. Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.Synonyms: END, ENG, HHT1, ORW, ORW1

Gene ID: 9606

UniProt: P17813