VEGFA antibody (N-Term)

Catalog No. ABIN115674

Product Details anti-VEGFA Antibody

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: The antibody will react with all VEGF-A isoforms.

Purification: Affinity Chromatography with immobilized Protein A

Immunogen: Highly purified N-terminal 20 amino acid peptide of Human VEGF-A

Application Details

Application Notes: ELISA: To detect Human VEGF by Direct ELISA a concentration of at least 0.5-2 μg/mL isrequired. This purified IgG, in combination with compatible secondary reagents, allows thedetection of 1,0- 2,5 ng/well of recombinant Human VEGF165 or other VEGF splice forms. Western Blot: 5-10 μg/mL. It will detect 25 ng/lane of recombinant human and murineVEGF-A under reducing conditions, dimers are detected at higher protein concentrations.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of > 0.5 mg/mL

Buffer: PBS, pH 7.4 containing no preservative

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized IgG is stable at 2-8 °C for at least one month and for longer when kept at -20 °C. When reconstituted the antibody is stable for at least six weeks at 2-4 °C.

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGF-A (VEGFA Products)

Background: VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

Gene ID: 7422

NCBI Accession: NP_001020537

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling