LYVE1 antibody (AA 24-231)

Catalog No. ABIN115663

Product Details anti-LYVE1 Antibody

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Binding Specificity: AA 24-231

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYVE1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody will detect LYVE-1 on the surface of lymphatic endothelial cells by Immunohistochemistry. It detects a 70 kDa LYVE-1 band in Western blotting. This antibody is not reactive with Mouse LYVE-1.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Antigen Affinity Chromatography

Immunogen: Highly pure recombinant Human soluble LYVE-1 produced in insect cells. It consists of amino acid 24 (Ser) to 232 (Gly) and is fused to a C-terminal His-tag (6xHis).

Application Details

Application Notes: Immunohistochemistry on Frozen and Paraffin Embedded Sections (1-5 μg/mL). Heat mediated antigen retrieval is recommended when staining paraffin embeddedsections. Western blot (1-2 μg/mL). ELISA (1-15 μg/mL). Flow Cytometry (3-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: 5 mM PBS, pH 7.2 without preservatives or stabilizers

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Protect from Light!

Target Details for LYVE1

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Alternative Name: LYVE-1 (LYVE1 Products)

Background: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosamino-glycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41 % similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 Molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 Molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels.Synonyms: CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, XLKD1

Gene ID: 10894

NCBI Accession: NP_006682

UniProt: Q9Y5Y7

Pathways: Glycosaminoglycan Metabolic Process