Ezrin antibody (AA 362-585)
(2 references)-
- Target See all Ezrin (EZR) Antibodies
- Ezrin (EZR)
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Binding Specificity
- AA 362-585
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Reactivity
- Human, Mouse, Rat, Cow, Hamster, Kangaroo
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Ezrin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
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This antibody reacts with a protein of 81 kDa.
Cellular Localization: Cell membrane - Purification
- Purified
- Immunogen
- BALB/C mice were immunized with a recombinant fragment of human ezrin corresponding to amino acid 362-585.
- Clone
- 3C12
- Isotype
- IgG1
- Top Product
- Discover our top product EZR Primary Antibody
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anti-Ezrin (EZR) (AA 292-464) antibody
EZR Reactivity: Human WB, ELISA, ICC, FACS Host: Mouse Monoclonal 6F1A9 unconjugated
anti-Ezrin (EZR) (AA 1-295) antibodyEZR Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) (pTyr478) antibodyEZR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) (AA 1-586) antibodyEZR Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) (pTyr353) antibodyEZR Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) antibodyEZR Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal CPTC-Ezrin-1 unconjugated
anti-Ezrin (EZR) (AA 1-586) antibodyEZR Reactivity: Human WB, ELISA, IHC (p), PLA Host: Mouse Monoclonal 1E11 unconjugated
anti-Ezrin (EZR) (AA 350-470) antibodyEZR Reactivity: Human WB, IP, IF Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) (AA 1-251) antibodyEZR Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) (AA 455-586) antibodyEZR Reactivity: Human, Rat WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Immunoprecipitation. Western blot: 1: 100-750. Immunohistochemistry on paraffin embedded sections: dilute 1: 50-1: 100 in an ABC method(30 minutes at room temperature). Recommended positive control: Lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "Knockdown of ezrin causes intrahepatic cholestasis by the dysregulation of bile fluidity in the bile duct epithelium in mice." in: Hepatology (Baltimore, Md.), Vol. 61, Issue 5, pp. 1660-71, (2015) (PubMed).
: "Polarization and migration of hematopoietic stem and progenitor cells rely on the RhoA/ROCK I pathway and an active reorganization of the microtubule network." in: The Journal of biological chemistry, Vol. 285, Issue 41, pp. 31661-71, (2010) (PubMed).
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: "Knockdown of ezrin causes intrahepatic cholestasis by the dysregulation of bile fluidity in the bile duct epithelium in mice." in: Hepatology (Baltimore, Md.), Vol. 61, Issue 5, pp. 1660-71, (2015) (PubMed).
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- Target
- Ezrin (EZR)
- Alternative Name
- Ezrin (EZR Products)
- Background
- Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. Ezrin is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.Synonyms: Cytovillin, EZR, VIL2, Villin-2, p81
- Gene ID
- 7430
- NCBI Accession
- NP_001104547
- UniProt
- P15311
- Pathways
- Maintenance of Protein Location
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