CDKN1C antibody

Catalog No. ABIN114825

Product Details anti-CDKN1C Antibody

Target: CDKN1C (Cyclin-Dependent Kinase Inhibitor 1C (p57, Kip2) (CDKN1C))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDKN1C antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody is specific to a protein of 57 kDa known as p57Kip2 a cell cycle regulatory mitotic inhibitor. It does not cross react with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes and is negative regulator of cell proliferation.

Purification: Ig Fraction

Immunogen: BALB/C mice were injected with mouse recombinant p57Kip2protein.

Clone: 57P06

Isotype: IgG2b

Application Details

Application Notes: Immunuprecipitation (Native). Immunohistochemistry on Paraffin Embedded Sections: 1/25-1/50 in an ABC method 30-60 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDKN1C

Target: CDKN1C (Cyclin-Dependent Kinase Inhibitor 1C (p57, Kip2) (CDKN1C))

Alternative Name: CDKN1C / KIP2 (CDKN1C Products)

Background: P57Kip2 (or CDKN1C) is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. The gene encoding human p57Kip2 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm's tumor, and Beckwith-Wiedemann syndrome (BWS), a cancer syndrome, making it a tumor suppressor candidate. BWS is characterized by numerous growth abnormalities and an increased risk of childhood tumors. Several types of childhood tumors including Wilms tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin-like growth factor II (IGF-II) and H19, both of which seem to be implicated in adrenal neoplasms.Synonyms: Cyclin-dependent kinase inhibitor 1C, Cyclin-dependent kinase inhibitor p57, p57KIP2

Gene ID: 1028

NCBI Accession: NP_000067

UniProt: P49918

Pathways: Cell Division Cycle, Dopaminergic Neurogenesis