KRT17 antibody

Catalog No. ABIN114619

Product Details anti-KRT17 Antibody

Target: KRT17 (Keratin 17 (KRT17))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT17 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Clone E3 reacts with Cytokeratin 17 in basal layers of pseudo-stratified and transitional epithelia.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat.

Purification: Purified

Immunogen: A cytoskeletal preparation from rat colon

Clone: Ks17-E3

Isotype: IgG2b

Application Details

Application Notes: Immunoblotting (1: 100 - 1: 1000). Immunocytochemistry. Immunohistochemistry on frozen and paraffin-embedded tissues (1: 25- 1: 200 forimmunohistochemistry with avidin-biotinylated horseradish peroxidase complec (ABC) asdetection regant). Flow cytometry (1: 25-1: 200)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KRT17

Target: KRT17 (Keratin 17 (KRT17))

Alternative Name: Cytokeratin 17 (KRT17 Products)

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immuno-histochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK17, Cytokeratin-17, K17, KRT17, Keratin 17, Keratin type I cytoskeletal 17, Keratin-17

Molecular Weight: 46 kDa

Gene ID: 3872

NCBI Accession: NP_000413

UniProt: Q04695