Smooth Muscle Actin antibody (N-Term)

Catalog No. ABIN114404

The Mouse Monoclonal anti-Smooth Muscle Actin antibody has been validated for IHC (p). It is suitable to detect Smooth Muscle Actin in samples from Human, Rat, Mouse, Chicken, Rabbit and Cow.

Quick Overview for Smooth Muscle Actin antibody (N-Term) (ABIN114404)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human, Rat, Mouse, Chicken, Rabbit, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1A4-asm-1

Product Details anti-Smooth Muscle Actin Antibody

Binding Specificity: N-Term

Specificity: This antibody is specific to alpha-smooth isoform of actin. It reacts with smooth muscle cells of vessels and different parenchymes. The antibody does not cross-react with beta and gamma-cytoplasmic, alpha-sarcomeric and alpha-myocardial actin isoforms.
Cellular Localization: Cytoplasmic.

Purification: Purified

Immunogen: N-terminal decapeptide of alpha-smooth muscle Actin

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on Formalin Fixed Paraffin Embedded Sections: Use Actin antibodyat 1/25-1/50 dilution in an ABC method for 30 minutes at room temperature. Recommended Positive Control: Leiomyoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Background: Smooth muscle actin is expressed in normal smooth muscle and in myoepithelial cells, and also in tumours derived from these cells. Actins are involved in maintaining the structure and integrity of a cell, as well as cell motility. They are highly conserved proteins and make up the majority of the contractile apparatus. To date, six different actin isoforms have been described.Synonyms: ACTSA, ACTVS, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

Gene ID: 59

NCBI Accession: NP_001135417

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development