MHC Class I (RT1Ac) antibody (PE)

Catalog No. ABIN114397

Product Details anti-MHC Class I (RT1Ac) Antibody (PE)

Target: MHC Class I (RT1Ac)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class I (RT1Ac) antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Purification: Protein G chromatography

Immunogen: PHA activated rat lymphocytes

Clone: OX-27

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water.

Buffer: PBS containing 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: Do Not Use Sodium Azide as preservative.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for MHC Class I (RT1Ac)

Target: MHC Class I (RT1Ac)

Abstract: MHC Class I (RT1Ac) Products

Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: MHC class I RT1.Ac heavy chain, RT1-A3

Gene ID: 10116

UniProt: Q31255