MHC Class I (RT1Ac) antibody (FITC)

Catalog No. ABIN114394

This Mouse Monoclonal antibody specifically detects MHC Class I (RT1Ac) in FACS. It exhibits reactivity toward Rat.

Quick Overview for MHC Class I (RT1Ac) antibody (FITC) (ABIN114394)

Target: MHC Class I (RT1Ac)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class I (RT1Ac) antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: OX-27

Product Details anti-MHC Class I (RT1Ac) Antibody (FITC)

Purification: Protein G Chromatography.

Immunogen: Phytohaemagglutinin Blasts.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: use 0.5 g of neat antibody to label 10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Add. Information: Recognizes a polymorphic determinant of the MHC class I antigen in the rat. This Antibody can be used for labelling cells of donor or host origin in bone marrowchimeras. (1,2)

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS buffer with 0.02 % sodium azide as preservative and 0.5 % EIA grade BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for MHC Class I (RT1Ac)

Target: MHC Class I (RT1Ac)

Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: MHC class I RT1.Ac heavy chain, RT1-A3

Gene ID: 10116

UniProt: Q31255