MHC Class II I-Ek antibody

Catalog No. ABIN114319

Product Details anti-MHC Class II I-Ek Antibody

Target: MHC Class II I-Ek

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II I-Ek antibody is un-conjugated

Application: Cytotoxicity Test (CyTox)

Purification: Protein G chromatography

Immunogen: C3H Donor: C3H.SW Fusion Partner: SP2/0-Ag 14

Clone: 14-4-4S

Isotype: IgG2a

Application Details

Application Notes: Cytotoxicity analysis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-Mb cell separation medium. After washing, adjust the cellconcentration to 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 1000 and mix.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS and 0.02 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MHC Class II I-Ek

Target: MHC Class II I-Ek

Abstract: MHC Class II I-Ek Products