MHC Class II I-Ap antibody
Reactivity: Mouse
FACS, CyTox
Host: Mouse
Monoclonal
7-16-17
unconjugated
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- Target
- MHC Class II I-Ap
- Reactivity
- Mouse
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
- Application
- Flow Cytometry (FACS), Cytotoxicity Test (CyTox)
- Purification
- Protein G chromatography
- Immunogen
- B10.p
- Clone
- 7-16-17
- Isotype
- IgG2a
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-
- Application Notes
-
Flow cytometry (protocol see below). Cytotoxic assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 2-1. 0 µg of antibody. 5. Vortex the tubes to ensure thorough mixing of antibody and cells.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- PBS and 0.02 % sodium azide (NaN3).
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- Target
- MHC Class II I-Ap
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