MHC Class II I-Abd antibody

Catalog No. ABIN114309

Product Details

Target: MHC Class II I-Abd

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Cytotoxicity Test (CyTox)

Purification: Affinity chromatography

Immunogen: C3H.SW spleen Donor: C3H Fusion Partner: SP2/0Ag.14

Clone: 28-16-8S

Isotype: IgM

Application Details

Application Notes: Flow Cytometry. Can be used to quantitate or eliminate cells bearing the I-Ab antigen from the appropriatestrains of mice.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-Mb cell separation medium. After washing, adjust the cellconcentration to 1. 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 640 and mix.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS and contains 0.02 % sodium azide (NaN3) as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: MHC Class II I-Abd