Neutrophils antibody (FITC)

Catalog No. ABIN114281

Product Details anti-Neutrophils Antibody (FITC)

Target: Neutrophils

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Neutrophils antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Immunogen: Cultured bone marrow cells.

Clone: 07-04-14

Isotype: IgG2a

Application Details

Application Notes: This antibody is suitable for use in Flow cytometry. This clone has also been reported to be useful for Immunohistochemistry (both frozen andparaffin sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation using a NH4Cl lysing buffer. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells,representing 1 test). 4. To each tube, add ~1. 0 μg of ABIN114280 per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes arelight sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 μl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl ofpropidium iodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS with 0.02 % sodium azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Neutrophils

Target: Neutrophils

Abstract: Neutrophils Products