CD5 antibody

Catalog No. ABIN114159

Product Details anti-CD5 Antibody

Target: CD5

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Cytotoxicity Test (CyTox)

Specificity: This anti-mouse CD5 (Ly 1.2) monoclonal antibody reacts with T cells from mouse strains expressing the Ly 1.2 phenotype. Can be used to identify T helper cells as they have a high density of Ly 1 on their surface.

Purification: Protein G Chromatography

Immunogen: C3H.CE - Ly 1.2 : DS. Donor: C3H spleen. Fusion Partner: Myeloma SP2/0 - Ag 14 (M5).

Clone: CG16

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry Analysis (see Protocols). Cytotoxic Depletion of CD5 Positive Lymphocytes (see Protocols). Functional Analysis (see Protocols). Cytotoxicity Analysis (see Protocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.02 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD5

Target: CD5

Alternative Name: CD5 (CD5 Products)

Background: CD5 is a 55 kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5

Gene ID: 12507

NCBI Accession: NP_031676

UniProt: P13379