Collagen IV antibody

Catalog No. ABIN114114

Product Details anti-Collagen IV Antibody

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Mouse, Orang-Utan

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: Antibody BP975 is specific for Mouse collagen type IV. The following cross reactivities have been observed: Mouse Type IV: 100 % Mouse Type III: < 0.1 % Mouse Type II: < 0.1 % Mouse Type I: < 0.1 % Human Type V: < 0.1 % Human Type IV: < 0.1 % Mouse Fibronectin: < 0.1 % Mouse Laminin: < 0.1 %

Cross-Reactivity (Details): Species reactivity (expected):OrangutanSpecies reactivity (tested):Mouse

Purification: Antigen Affinity Chromatography

Immunogen: Collagen IV purified from Mouse EHS tumour

Application Details

Application Notes: Immunohistochemistry on Paraffin Embedded Sections (1/250-1/1,000): Antigen retrievalis not necessary but may improve results, the recommended fixatives are AFA, 5-10 % buffered formalin or Bouin's. Immunofluorescence (1/40-1/60). ELISA (1/100-1/400).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store undiluted (in aliquots) at -20 °C.

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody

Background: Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.Synonyms: COL4A1

Gene ID: 12826

NCBI Accession: NP_034061

UniProt: P02463