FITC antibody
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- Target See all FITC products
- FITC (Fluorescein Isothiocyanate (FITC))
- Reactivity
- Please inquire
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FITC antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Purification
- Immunoaffinity Chromatography.
- Immunogen
- FITC-KLH conjugate
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anti-Fluorescein Isothiocyanate (FITC) antibody
FITC ELISA, WB, IHC, FACS Host: Sheep Polyclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibody (HRP)FITC ELISA, WB, IHC Host: Sheep Polyclonal HRP
anti-Fluorescein Isothiocyanate (FITC) antibody (Alkaline Phosphatase (AP))FITC ELISA, WB, IHC Host: Sheep Polyclonal Alkaline Phosphatase (AP)
anti-Fluorescein Isothiocyanate (FITC) antibody (Biotin)FITC ELISA, WB, IHC, FACS Host: Sheep Polyclonal Biotin
anti-Fluorescein Isothiocyanate (FITC) antibody (PE)FITC ELISA, FACS Host: Sheep Polyclonal PE
anti-Fluorescein Isothiocyanate (FITC) antibodyFITC WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Suitable for use in ELISA and Western blot. It is also suitable for conjugation purposes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL (OD280nm, E0.1% = 1.4)
- Buffer
- 0.01 M PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and without stabilizing proteins.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluited at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- FITC (Fluorescein Isothiocyanate (FITC))
- Alternative Name
- FITC (FITC Products)
- Target Type
- Chemical
- Background
- FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.Synonyms: Fluorescein Isothiocyanate
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