FITC antibody (HRP)

Catalog No. ABIN113423

Product Details anti-FITC Antibody (HRP)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FITC antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Characteristics: Molar Ratio: HRP:IgG is >1.5

Purification: Solid Phase hapten-antibodies capture.

Immunogen: FITC-KLH conjugate

Application Details

Application Notes: Suitable for use in Western Blot and ELISA. Recommended Chromogens: Diaminobenzidine or TMB for immunohistochemistry,4-chloro napthol for Immunoblotting and0-phenylenediamine or ABTS for Enzymeimmunoassay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1-2 mg/mL (OD280)

Buffer: Saline Phosphate containing 0.02 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized product at 2 - 8 °C for up to one month or at -20 °C for longer. Following reconstitution store in aliquots at -20 °C.

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC (FITC Products)

Target Type: Chemical

Background: FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.Synonyms: Fluorescein Isothiocyanate