H. pylori
Reactivity: Helicobacter pylori
ELISA, WB, IHC, FM
Host: Rabbit
Polyclonal
Biotin
Application Notes
Immunohistochemistry (formalin-fixed paraffin embedded). ELISA (> 1/5,000). Immunofluorescence microscopy (> 1/1,000). Acetone fixation of the antigen source is recommended prior to staining. Enzyme amplification following reaction with FITC conjugate can also be accomplishedutilizing enzyme-antibody conjugates specific to FITC. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
4-5 mg/mL (OD280 nm, E0.1%= 1.4)
Buffer
0.01 M PBS, pH 7.2, containing 10 mg/mL BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. More recently this bacterium has also been implicated with a number of vascular disorders including heart disease. It is not clear how H. pylori is transmitted or why some patients become symptomatic while others do not. The bacteria are most likely spread from person to person through fecal-to-oral or oral-to-oral routes. Possible environmental reservoirs include contaminated water sources.Synonyms: H. pylori