E. Coli antibody (FITC)
Reactivity: E. coli
IF
Host: Goat
Polyclonal
FITC
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- Target See all E. Coli products
- E. Coli
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Reactivity
- E. coli
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This E. Coli antibody is conjugated to FITC
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Application
- Immunofluorescence (IF)
- Immunogen
- Mixture of E. coli serotypes
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- Application Notes
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Indirect Immunofluorescence (titer >1: 1,600) assay vs serotypes O157: H7, O20, O125, O55,O111 and K12. Direct Fluorescenceimmunoassay staining of target antigens in a permissive tissue culturesystem. Acetone fixation of the antigen source is recommended prior to staining. Enzymeamplification following reaction with FITC conjugate can also be accomplished utilizingenzyme-antibody conjugates specific to FITC.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 4-5 mg/mL (OD280 nm, E 0.1% = 0 1.4)
- Buffer
- 0.01 M PBS, pH 7.2 with 0.1 % sodium azide as preservative and 10 mg/mL BSA as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions
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- Target
- E. Coli
- Abstract
- E. Coli Products
- Target Type
- Bacteria
- Background
- Synonyms: EHEC, EPEC, ETEC, Escherichia coli
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