E. Coli antibody (Biotin)
Reactivity: E. coli
IF, WB, EIA
Host: Rabbit
Polyclonal
Biotin
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- Target See all E. Coli products
- E. Coli
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Reactivity
- E. coli
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This E. Coli antibody is conjugated to Biotin
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Application
- Immunofluorescence (IF), Western Blotting (WB), Enzyme Immunoassay (EIA)
- Purification
- Protein A Chromatography.
- Immunogen
- Mixture of E. coli serotypes
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- Application Notes
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Avidin and streptavidin amplification systems for ELISA and Fluorescence Microscopy. Has been described for previous lots to work in Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 4-5 mg/mL (OD280 nm, E0.1% = 1.4)
- Buffer
- 0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and no stabilizers.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- E. Coli
- Abstract
- E. Coli Products
- Target Type
- Bacteria
- Background
- Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.Synonyms: EHEC, EPEC, ETEC, Escherichia coli
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