E. Coli antibody
Reactivity: E. coli
IF, EIA
Host: Rabbit
Polyclonal
unconjugated
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- Target See all E. Coli products
- E. Coli
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Reactivity
- E. coli
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This E. Coli antibody is un-conjugated
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Application
- Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- Purification
- Protein A Chromatography.
- Immunogen
- Mixture of E. coli serotypes.
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- Application Notes
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ELISA (>1: 2,000 by ELISA vs serotypes O157: H7, O20, O125, O55, O111 and K12). Fluorescence MicroscopyConjugation. May be used in place of whole antiserum in almost any appropriate antibody basedtechnique.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 4-5 mg/mL (OD280 nm, E0.1% = 1.4)
- Buffer
- 0.01 M PBS pH 7.2 containing 0.09 % Sodium Azide as a preservative and no stabilizing proteins.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- E. Coli
- Abstract
- E. Coli Products
- Target Type
- Bacteria
- Background
- Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.Synonyms: EHEC, EPEC, ETEC, Escherichia coli
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