Suitable for use in ELISA, IFA (1/1.200), Immunohistochemistry, Double Diffusion and CIE. Also suitable for conjugation purposes. Use neat in gel-precipitin reactions. Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
4-5 mg/mL (OD280 nm, E0.1% = 1.4)
Buffer
0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative without stabilizing proteins
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Goyer, Loiselet, Bon, LOllivier, Laue, Holland, Bonnin, Dalle: "Intestinal Cell Tight Junctions Limit Invasion of Candida albicans through Active Penetration and Endocytosis in the Early Stages of the Interaction of the Fungus with the Intestinal Barrier." in: PLoS ONE, Vol. 11, Issue 3, pp. e0149159, (2016) (PubMed).
Schild, Heyken, de Groot, Hiller, Mock, de Koster, Horn, Rupp, Hube: "Proteolytic cleavage of covalently linked cell wall proteins by Candida albicans Sap9 and Sap10." in: Eukaryotic cell, Vol. 10, Issue 1, pp. 98-109, (2011) (PubMed).
Candida albicans is the most frequently isolated fungal pathogen of Humans, affecting immunocompromised patients ranging from premature infants to AIDS sufferers. Systemic infections have an attributed mortality of 30-50 % . C. albicans is a diploid organism which has eight sets of homologous chromosomes. It has a genome of approximately 16 Mb (haploid), about 30 % greater than S. cerevisiae (baker's yeast).